D3922 bodipy

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August undefined, 2024

WebMay 14, 2024 · To analyze their lipid droplet formation for the 3D culture, the organoids were transferred to 6 super-low attachment well dishes, incubated in 0.2% BODIPY (# D3922, … WebCell-cultured meat offers and potential in a more sustainable, ethical, resilient, and healthy food system. Does, research and development has been hindered through which lack of serum-free media which authorize to robust expansion of relevant cells (e.g., muscle satellite cells) over multiple passages. Recently, a low-cost serum-free media (B8) was described …

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WebBODIPY™ 493/503 can be used as a stain for neutral lipds, and as a tracer for oil and other nonpolar lipids. Shop Invitrogen™ BODIPY™ 493/503 (4,4 ... Supplier: Invitrogen™ … WebAfter treatment, cells were washed with PBS and fixed with 3.7% paraformaldehyde. Next, cells were stained with 2 μg/mL BODIPY 493/503 (D3922; Invitrogen, Thermo Fisher Scientific) and ActinRed555 (R37112; Thermo Fisher Scientific) and mounted with Vectashield Antifade Mounting Medium (H-1000-10; Vector Laboratories). shurlawn

Fluopack screening platform for unbiased cellular phenotype …

WebJul 27, 2024 · Appropriate secondary antibodies were purchased from Invitrogen. BODIPY (493/503) was used to fluorescently label lipid droplets (2 μM, cat. #D3922, Invitrogen). 7-Aminoactinomycin D (7AAD, 0.5 μg/ml, Thermo Fisher Scientific) was used to assess cellular viability. Sulfo-N-succinimidyl oleate (SSO ... WebAug 23, 2012 · Fluorophores such as BODIPY-labeled arachidonic acid or BODIPY-conjugated electrophiles can be paired with organelle-specific probes to identify specific biological processes and signaling pathways activated in ... D3922: BODIPY FL *-503: 510-Invitrogen: D2390: BODIPY-FL-IA: Free thiols: 507: 510-Invitrogen: D6003: BODIPY … WebBODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) a Biochemical by Invitrogen Antibodies ... shur law cincinnati

Maternal obesity alters placental lysophosphatidylcholines, lipid ...

BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora …

WebAug 14, 2024 · Cells were subsequently cultured with or without γ + LPS for 18 h, followed by staining with Bodipy 493/503 (#D3922, ThermoFisher, 2 μM) for 1 h, fixation in 2% … WebMar 29, 2024 · Further histological staining of TH+ neurons with BODIPY, a dye that specifically labels neutral lipids and is commonly used to detect LDs [30], showed that both the number and size of BODIPY+LDs in the SNpc were higher in PD mice than in controls, and were significantly decreased by Ka treatment (Fig. 3f-h). the overtimerWebBODIPY FL Dye. Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a … the overtime shift

"WebFeb 7, 2024 · D3922: BODIPY 493: Lipid metabolism and trafficking: Neutral lipids: H34475: HCS LipidTOX Green: Lipid metabolism and trafficking: Neutral lipids: D3921: BODIPY 505: Lipid ... (BODIPY FL C5, C12 and C16) ranked among the top 15 probes, as well as all probes staining endogenous neutral lipids (BODIPY 493, ... " - D3922 bodipy

D3922 bodipy

BODIPY™ 493/503（4,4-二氟-1,3,5,7,8-五甲基-4-硼杂-3a,4a-二 …

WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 … WebBODIPY™ 493/503 staining Thermo Scientific CAT: D3922 Lipofectamine 2000 Thermo Scientific CAT: 11668019 Pierce™ BCA Protein Assay Kit Thermo Scientific CAT: 23227 MG132 Selleck CAT: S2619 Chloroquine diphosphate Selleck CAT: S4157 Triglyceride (TG) Nanjing Jiancheng Bioengineering Institute CAT: A110-1 Total cholesterol (TC) Nanjing …

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WebApr 13, 2024 · Cells were incubated with PBS buffer containing 0.01 mg/mL DAPI (Thermo Fisher, 62248) and 2 μM BODIPY (Thermo Fisher, D3922) for 5 min before mounting in VECTASHIELD Antifade Mounting Medium. To quantify the fluorescence intensity of Plin2 or BODIPY in cells, images were taken with the same confocal set-up. WebBODIPY analog (Invitrogen # D3922, Carlsbad, CA, USA) was added to 10 mL of the 5% chicken egg solution and vortexed for 30 s for a final concentration of 6.4 µM of BODIPY-FL. Twenty 5 dpf larvae were incubated in 4 mL of the chicken egg solution for 2 h in the dark at 28.5 °C and washed in egg water following feeding.

WebBackground Regulating T-cell metabolism is crucial for their anticancer activity. Therefore, understanding the function and metabolism of human tumor-infiltrating T cells is of broad interest and clinical importance. Methods CD3+CD45+ T cells were sorted from adjacent area or tumor core of human hepatocellular carcinoma (HCC), then the clusters and … http://www.maokangbio.com/productView.action?id=10540

WebLiver and placenta BODIPY staining. Serial transverse sections (10 μm) of cryopreserved dam livers and placentas were adhered to slides. ... (liver) or 1:1000 (placenta) dilution of BODIPY 493/503 (Thermofisher D3922) in PBS for 20 min. Slides were rinsed three times in PBS for 5 min, ... WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene)ethyl]-3,5-dimethyl-1H-pyrrolato-N1,N2]difluoro-, (T-4)-Molecular Formula C14H17BF2N2 Molecular Weight 262.11 Appearance red solid Lot …

WebD3922 Print Invitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) Nonpolar structure. Supplier: Invitrogen™ D3922 ... BODIPY™ …

WebJun 28, 2024 · BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Prepare 5 mM BODIPY stock solution Dissolve 1.3 mg … shur law co lpaWebAfter 48 hours, cells were stained by BODIPY™ FL C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific #D3922) to determine the amount of lipid droplet in regular conditions. We added 200µM Oleic Acid conjugated with BSA into cells and incubated for overnight. shurley adverb jingleWebSep 5, 2016 · In this protocol, we describe the use of the fluorescent neutral lipid dye 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) to … shur law co. lpaWebJul 1, 2007 · Protocols for the detection of lipid droplets with nile red and BODIPY 493/503 are included. ... Molecular Probes #D3922) Dissolve in 5 ml ethanol . Aliquot and store at … shur law group shurleendorough gmail.comWebCatalog number: D3922. With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer … the overton brentwoodWebJun 11, 2024 · Adult zebrafish were placed in tanks with 10 ng/µl BODIPY 493/503 (Thermo Fisher, Waltham, USA; catalog #D3922) for 30 min in the dark. Fish were washed and then placed in new tanks with fresh water for 2 hr. Fish were washed again to remove any residual BODIPY, then anesthetized and imaged as indicated above for whole adipose … the overton at brentwood